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p jak2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p jak2
    P Jak2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+jak2+antibody/Jak2+XP+Rabbit+mAb/pm41910173-335-34-36
    Average 96 stars, based on 1265 article reviews
    p jak2 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Western Blot:

    Article Title: BAG3 promotes proliferation and migration of arterial smooth muscle cells by regulating STAT3 phosphorylation in diabetic vascular remodeling
    Article Snippet: .. Rabbit-anti-BAG3 antibody (10599-1-AP, Proteintech, WB: 1:2000; IP: 1:1000; IF: 1:100), rabbit-anti-MMP2 antibody (10373-2-AP, Proteintech, WB: 1:1000), rabbit-anti-MMP9 antibody (10375-2-AP, Proteintech, WB: 1:1000), rabbit-anti-PCNA antibody (10205-2-AP, Proteintech, WB: 1:1000), mouse-anti-Tubulin antibody (66031-1-Ig, Proteintech, WB: 1:1000), mouse-anti-GAPDH antibody (60004-1-Ig, Proteintech, WB: 1:1000), mouse-anti-Flag antibody (66008-4-Ig, Proteintech, WB: 1:1000; IP: 1:1000), rabbit-anti-JAK2 antibody (3230 S, CST, WB: 1:1000), rabbit-anti-p-JAK2 antibody (3776 S, CST, WB: 1:1000), mouse-anti-STAT3 antibody (9139 S, CST, WB: 1:1000; IP: 1:1000), rabbit-anti-p-STAT3(705) antibody (9145 S, CST, WB: 1:1000; IF: 1:100), rabbit-anti-p-STAT3(727) antibody (94,994 S, CST, WB: 1:1000; IF: 1:100), mouse-anti-ERK1/2 antibody (sc-514,302, Santa, WB: 1:1000), mouse-anti-p-ERK1/2 antibody (sc-81,492, Santa, WB: 1:1000), mouse-anti-GATA3 antibody (66400-1-Ig, Proteintech, WB: 1:1000), HRP goat-anti-rabbit IgG antibody (A21020, Abbkine, WB: 1:10000), HRP goat-anti-mouse IgG antibody (A21010, Abbkine, WB: 1:10000), Rhodamine(TRITIC) conjugated goat-anti-rabbit IgG(H + L) (SA00007-2, Proteintech, IF: 1:200), Protein A/G magnetic Beads (Cat#B23202, Biotool), AntiFade Mounting Medium (Beyotime), CCK-8 cell viability kit (B34304, Bimake), Higene transfection reagent (C1506, APPLYGEN), Jet kit (101,000,046, Polyplus), protease inhibitor (B14001, Bimake), phosphatase inhibitors (B15001, Bimake), SH-4-54 (S7337, Selleck), U0126-EtOH (S1102, Selleck). ..

    Article Title: Effect of spirocyclopiperazinium salt compound LXM-15 on spinal nerve injury in rats.
    Article Snippet: Eur J Pain.. 2024;28:297–309. wileyonlinelibrary.com/journal/ejp | 297© 2023 European Pain Federation - EFIC ®.. Tian Yu Wang and Ying Ying Liang contributed equally to this work.

    Article Title: BAG3 promotes proliferation and migration of arterial smooth muscle cells by regulating STAT3 phosphorylation in diabetic vascular remodeling
    Article Snippet: .. 2.17 Antibodies and reagents Page 7/24 Rabbit-anti-BAG3 antibody (10599-1-AP, Proteintech, WB: 1:2000; IP: 1:1000; IF: 1:100), rabbit-anti-MMP2 antibody (10373-2-AP, Proteintech, WB: 1:1000), rabbit-anti-MMP9 antibody (10375-2-AP, Proteintech, WB: 1:1000), rabbit-anti-PCNA antibody (10205-2-AP, Proteintech, WB: 1:1000), mouse-anti-Tubulin antibody (66031-1-Ig, Proteintech, WB: 1:1000), mouse-anti-GAPDH antibody (60004-1-Ig, Proteintech, WB: 1:1000), mouse-anti-Flag antibody (66008-4-Ig, Proteintech, WB: 1:1000; IP: 1:1000), rabbit-anti-JAK2 antibody (3230S, CST, WB: 1:1000), rabbit-anti-p-JAK2 antibody (3776S, CST, WB: 1:1000), mouse-anti-STAT3 antibody (9139S, CST, WB: 1:1000; IP: 1:1000), rabbit-anti-p-STAT3(705) antibody (9145S, CST, WB: 1:1000), rabbit-anti-p-STAT3(727) antibody (94994S, CST, WB: 1:1000), mouse-anti-ERK1/2 antibody (sc514302, Santa, WB: 1:1000), mouse-anti-p-ERK1/2 antibody (sc-81492, Santa, WB: 1:1000), mouse-antiGATA3 antibody (66400-1-Ig, Proteintech, WB: 1:1000), HRP goat-anti-rabbit IgG antibody (A21020, Abbkine, WB: 1:10000), HRP goat-anti-mouse IgG antibody (A21010, Abbkine, WB: 1:10000), Rhodamine(TRITIC) conjugated goat-anti-rabbit IgG(H + L) (SA00007-2, Proteintech, IF: 1:200), Protein A/G magnetic Beads (Cat#B23202, Biotool), AntiFade Mounting Medium (Beyotime), CCK-8 cell viability kit (B34304, Bimake), Higene transfection reagent (C1506, APPLYGEN), Jet kit (101000046, Polyplus), protease inhibitor (B14001, Bimake), phosphatase inhibitors (B15001, Bimake), SH-4-54 (S7337, Selleck), U0126-EtOH (S1102, Selleck). ..

    Article Title: BAG3 promotes proliferation and migration of arterial smooth muscle cells by regulating STAT3 phosphorylation in diabetic vascular remodeling.
    Article Snippet: .. Rabbit-anti-BAG3 antibody (10599-1-AP, Proteintech, WB: 1:2000; IP: 1:1000; IF: 1:100), rabbit-anti-MMP2 antibody (10373-2-AP, Proteintech, WB: 1:1000), rabbit-anti-MMP9 antibody (10375-2-AP, Proteintech, WB: 1:1000), rabbit-anti-PCNA antibody (10205-2-AP, Proteintech, WB: 1:1000), mouse-anti-Tubulin antibody (66031-1-Ig, Proteintech, WB: 1:1000), mouse-antiGAPDH antibody (60004-1-Ig, Proteintech, WB: 1:1000), mouse-anti-Flag antibody (66008-4-Ig, Proteintech, WB: 1:1000; IP: 1:1000), rabbit-anti-JAK2 antibody (3230 S, CST, WB: 1:1000), rabbit-anti-p-JAK2 antibody (3776 S, CST, WB: 1:1000), mouse-anti-STAT3 antibody (9139 S, CST, WB: 1:1000; IP: 1:1000), rabbit-anti-p-STAT3(705) antibody (9145 S, CST, WB: 1:1000; IF: 1:100), rabbit-anti-p-STAT3(727) antibody (94,994 S, CST, WB: 1:1000; IF: 1:100), mouse-anti-ERK1/2 antibody (sc514,302, Santa, WB: 1:1000), mouse-anti-p-ERK1/2 antibody (sc-81,492, Santa, WB: 1:1000), mouse-anti-GATA3 antibody (66400-1-Ig, Proteintech, WB: 1:1000), HRP goat-anti-rabbit IgG antibody (A21020, Abbkine, WB: 1:10000), HRP goat-anti-mouse IgG antibody (A21010, Abbkine, WB: 1:10000), Rhodamine(TRITIC) conjugated goat-anti-rabbit IgG(H + L) (SA00007-2, Proteintech, IF: 1:200), Protein A/G magnetic Beads (Cat#B23202, Biotool), AntiFade Mounting Medium (Beyotime), CCK-8 cell viability kit (B34304, Bimake), Higene transfection reagent (C1506, APPLYGEN), Jet kit (101,000,046, Polyplus), protease inhibitor (B14001, Bimake), phosphatase inhibitors (B15001, Bimake), SH-4-54 (S7337, Selleck), U0126-EtOH (S1102, Selleck). ..

    Magnetic Beads:

    Article Title: BAG3 promotes proliferation and migration of arterial smooth muscle cells by regulating STAT3 phosphorylation in diabetic vascular remodeling
    Article Snippet: .. Rabbit-anti-BAG3 antibody (10599-1-AP, Proteintech, WB: 1:2000; IP: 1:1000; IF: 1:100), rabbit-anti-MMP2 antibody (10373-2-AP, Proteintech, WB: 1:1000), rabbit-anti-MMP9 antibody (10375-2-AP, Proteintech, WB: 1:1000), rabbit-anti-PCNA antibody (10205-2-AP, Proteintech, WB: 1:1000), mouse-anti-Tubulin antibody (66031-1-Ig, Proteintech, WB: 1:1000), mouse-anti-GAPDH antibody (60004-1-Ig, Proteintech, WB: 1:1000), mouse-anti-Flag antibody (66008-4-Ig, Proteintech, WB: 1:1000; IP: 1:1000), rabbit-anti-JAK2 antibody (3230 S, CST, WB: 1:1000), rabbit-anti-p-JAK2 antibody (3776 S, CST, WB: 1:1000), mouse-anti-STAT3 antibody (9139 S, CST, WB: 1:1000; IP: 1:1000), rabbit-anti-p-STAT3(705) antibody (9145 S, CST, WB: 1:1000; IF: 1:100), rabbit-anti-p-STAT3(727) antibody (94,994 S, CST, WB: 1:1000; IF: 1:100), mouse-anti-ERK1/2 antibody (sc-514,302, Santa, WB: 1:1000), mouse-anti-p-ERK1/2 antibody (sc-81,492, Santa, WB: 1:1000), mouse-anti-GATA3 antibody (66400-1-Ig, Proteintech, WB: 1:1000), HRP goat-anti-rabbit IgG antibody (A21020, Abbkine, WB: 1:10000), HRP goat-anti-mouse IgG antibody (A21010, Abbkine, WB: 1:10000), Rhodamine(TRITIC) conjugated goat-anti-rabbit IgG(H + L) (SA00007-2, Proteintech, IF: 1:200), Protein A/G magnetic Beads (Cat#B23202, Biotool), AntiFade Mounting Medium (Beyotime), CCK-8 cell viability kit (B34304, Bimake), Higene transfection reagent (C1506, APPLYGEN), Jet kit (101,000,046, Polyplus), protease inhibitor (B14001, Bimake), phosphatase inhibitors (B15001, Bimake), SH-4-54 (S7337, Selleck), U0126-EtOH (S1102, Selleck). ..

    Article Title: BAG3 promotes proliferation and migration of arterial smooth muscle cells by regulating STAT3 phosphorylation in diabetic vascular remodeling
    Article Snippet: .. 2.17 Antibodies and reagents Page 7/24 Rabbit-anti-BAG3 antibody (10599-1-AP, Proteintech, WB: 1:2000; IP: 1:1000; IF: 1:100), rabbit-anti-MMP2 antibody (10373-2-AP, Proteintech, WB: 1:1000), rabbit-anti-MMP9 antibody (10375-2-AP, Proteintech, WB: 1:1000), rabbit-anti-PCNA antibody (10205-2-AP, Proteintech, WB: 1:1000), mouse-anti-Tubulin antibody (66031-1-Ig, Proteintech, WB: 1:1000), mouse-anti-GAPDH antibody (60004-1-Ig, Proteintech, WB: 1:1000), mouse-anti-Flag antibody (66008-4-Ig, Proteintech, WB: 1:1000; IP: 1:1000), rabbit-anti-JAK2 antibody (3230S, CST, WB: 1:1000), rabbit-anti-p-JAK2 antibody (3776S, CST, WB: 1:1000), mouse-anti-STAT3 antibody (9139S, CST, WB: 1:1000; IP: 1:1000), rabbit-anti-p-STAT3(705) antibody (9145S, CST, WB: 1:1000), rabbit-anti-p-STAT3(727) antibody (94994S, CST, WB: 1:1000), mouse-anti-ERK1/2 antibody (sc514302, Santa, WB: 1:1000), mouse-anti-p-ERK1/2 antibody (sc-81492, Santa, WB: 1:1000), mouse-antiGATA3 antibody (66400-1-Ig, Proteintech, WB: 1:1000), HRP goat-anti-rabbit IgG antibody (A21020, Abbkine, WB: 1:10000), HRP goat-anti-mouse IgG antibody (A21010, Abbkine, WB: 1:10000), Rhodamine(TRITIC) conjugated goat-anti-rabbit IgG(H + L) (SA00007-2, Proteintech, IF: 1:200), Protein A/G magnetic Beads (Cat#B23202, Biotool), AntiFade Mounting Medium (Beyotime), CCK-8 cell viability kit (B34304, Bimake), Higene transfection reagent (C1506, APPLYGEN), Jet kit (101000046, Polyplus), protease inhibitor (B14001, Bimake), phosphatase inhibitors (B15001, Bimake), SH-4-54 (S7337, Selleck), U0126-EtOH (S1102, Selleck). ..

    Article Title: BAG3 promotes proliferation and migration of arterial smooth muscle cells by regulating STAT3 phosphorylation in diabetic vascular remodeling.
    Article Snippet: .. Rabbit-anti-BAG3 antibody (10599-1-AP, Proteintech, WB: 1:2000; IP: 1:1000; IF: 1:100), rabbit-anti-MMP2 antibody (10373-2-AP, Proteintech, WB: 1:1000), rabbit-anti-MMP9 antibody (10375-2-AP, Proteintech, WB: 1:1000), rabbit-anti-PCNA antibody (10205-2-AP, Proteintech, WB: 1:1000), mouse-anti-Tubulin antibody (66031-1-Ig, Proteintech, WB: 1:1000), mouse-antiGAPDH antibody (60004-1-Ig, Proteintech, WB: 1:1000), mouse-anti-Flag antibody (66008-4-Ig, Proteintech, WB: 1:1000; IP: 1:1000), rabbit-anti-JAK2 antibody (3230 S, CST, WB: 1:1000), rabbit-anti-p-JAK2 antibody (3776 S, CST, WB: 1:1000), mouse-anti-STAT3 antibody (9139 S, CST, WB: 1:1000; IP: 1:1000), rabbit-anti-p-STAT3(705) antibody (9145 S, CST, WB: 1:1000; IF: 1:100), rabbit-anti-p-STAT3(727) antibody (94,994 S, CST, WB: 1:1000; IF: 1:100), mouse-anti-ERK1/2 antibody (sc514,302, Santa, WB: 1:1000), mouse-anti-p-ERK1/2 antibody (sc-81,492, Santa, WB: 1:1000), mouse-anti-GATA3 antibody (66400-1-Ig, Proteintech, WB: 1:1000), HRP goat-anti-rabbit IgG antibody (A21020, Abbkine, WB: 1:10000), HRP goat-anti-mouse IgG antibody (A21010, Abbkine, WB: 1:10000), Rhodamine(TRITIC) conjugated goat-anti-rabbit IgG(H + L) (SA00007-2, Proteintech, IF: 1:200), Protein A/G magnetic Beads (Cat#B23202, Biotool), AntiFade Mounting Medium (Beyotime), CCK-8 cell viability kit (B34304, Bimake), Higene transfection reagent (C1506, APPLYGEN), Jet kit (101,000,046, Polyplus), protease inhibitor (B14001, Bimake), phosphatase inhibitors (B15001, Bimake), SH-4-54 (S7337, Selleck), U0126-EtOH (S1102, Selleck). ..

    CCK-8 Assay:

    Article Title: BAG3 promotes proliferation and migration of arterial smooth muscle cells by regulating STAT3 phosphorylation in diabetic vascular remodeling
    Article Snippet: .. Rabbit-anti-BAG3 antibody (10599-1-AP, Proteintech, WB: 1:2000; IP: 1:1000; IF: 1:100), rabbit-anti-MMP2 antibody (10373-2-AP, Proteintech, WB: 1:1000), rabbit-anti-MMP9 antibody (10375-2-AP, Proteintech, WB: 1:1000), rabbit-anti-PCNA antibody (10205-2-AP, Proteintech, WB: 1:1000), mouse-anti-Tubulin antibody (66031-1-Ig, Proteintech, WB: 1:1000), mouse-anti-GAPDH antibody (60004-1-Ig, Proteintech, WB: 1:1000), mouse-anti-Flag antibody (66008-4-Ig, Proteintech, WB: 1:1000; IP: 1:1000), rabbit-anti-JAK2 antibody (3230 S, CST, WB: 1:1000), rabbit-anti-p-JAK2 antibody (3776 S, CST, WB: 1:1000), mouse-anti-STAT3 antibody (9139 S, CST, WB: 1:1000; IP: 1:1000), rabbit-anti-p-STAT3(705) antibody (9145 S, CST, WB: 1:1000; IF: 1:100), rabbit-anti-p-STAT3(727) antibody (94,994 S, CST, WB: 1:1000; IF: 1:100), mouse-anti-ERK1/2 antibody (sc-514,302, Santa, WB: 1:1000), mouse-anti-p-ERK1/2 antibody (sc-81,492, Santa, WB: 1:1000), mouse-anti-GATA3 antibody (66400-1-Ig, Proteintech, WB: 1:1000), HRP goat-anti-rabbit IgG antibody (A21020, Abbkine, WB: 1:10000), HRP goat-anti-mouse IgG antibody (A21010, Abbkine, WB: 1:10000), Rhodamine(TRITIC) conjugated goat-anti-rabbit IgG(H + L) (SA00007-2, Proteintech, IF: 1:200), Protein A/G magnetic Beads (Cat#B23202, Biotool), AntiFade Mounting Medium (Beyotime), CCK-8 cell viability kit (B34304, Bimake), Higene transfection reagent (C1506, APPLYGEN), Jet kit (101,000,046, Polyplus), protease inhibitor (B14001, Bimake), phosphatase inhibitors (B15001, Bimake), SH-4-54 (S7337, Selleck), U0126-EtOH (S1102, Selleck). ..

    Article Title: BAG3 promotes proliferation and migration of arterial smooth muscle cells by regulating STAT3 phosphorylation in diabetic vascular remodeling
    Article Snippet: .. 2.17 Antibodies and reagents Page 7/24 Rabbit-anti-BAG3 antibody (10599-1-AP, Proteintech, WB: 1:2000; IP: 1:1000; IF: 1:100), rabbit-anti-MMP2 antibody (10373-2-AP, Proteintech, WB: 1:1000), rabbit-anti-MMP9 antibody (10375-2-AP, Proteintech, WB: 1:1000), rabbit-anti-PCNA antibody (10205-2-AP, Proteintech, WB: 1:1000), mouse-anti-Tubulin antibody (66031-1-Ig, Proteintech, WB: 1:1000), mouse-anti-GAPDH antibody (60004-1-Ig, Proteintech, WB: 1:1000), mouse-anti-Flag antibody (66008-4-Ig, Proteintech, WB: 1:1000; IP: 1:1000), rabbit-anti-JAK2 antibody (3230S, CST, WB: 1:1000), rabbit-anti-p-JAK2 antibody (3776S, CST, WB: 1:1000), mouse-anti-STAT3 antibody (9139S, CST, WB: 1:1000; IP: 1:1000), rabbit-anti-p-STAT3(705) antibody (9145S, CST, WB: 1:1000), rabbit-anti-p-STAT3(727) antibody (94994S, CST, WB: 1:1000), mouse-anti-ERK1/2 antibody (sc514302, Santa, WB: 1:1000), mouse-anti-p-ERK1/2 antibody (sc-81492, Santa, WB: 1:1000), mouse-antiGATA3 antibody (66400-1-Ig, Proteintech, WB: 1:1000), HRP goat-anti-rabbit IgG antibody (A21020, Abbkine, WB: 1:10000), HRP goat-anti-mouse IgG antibody (A21010, Abbkine, WB: 1:10000), Rhodamine(TRITIC) conjugated goat-anti-rabbit IgG(H + L) (SA00007-2, Proteintech, IF: 1:200), Protein A/G magnetic Beads (Cat#B23202, Biotool), AntiFade Mounting Medium (Beyotime), CCK-8 cell viability kit (B34304, Bimake), Higene transfection reagent (C1506, APPLYGEN), Jet kit (101000046, Polyplus), protease inhibitor (B14001, Bimake), phosphatase inhibitors (B15001, Bimake), SH-4-54 (S7337, Selleck), U0126-EtOH (S1102, Selleck). ..

    Article Title: Effect of scutellarin on BV-2 microglial-mediated apoptosis in PC12 cells via JAK2/STAT3 signalling pathway.
    Article Snippet: .. Scutellarin (#THT033, Ronghe Medical Technology, Shanghai, China), F12/DMEM 1:1 high sugar medium (#C3130-0500, BI, Shanghai, China ), sugar-free culture medium (#C3122-500, BI, Shanghai, China), cell culture level anhydrous glucose (#G8150, Solarbio, Beijing, China), TUNEL kit (#PF00006, Proteintech, Wuhan, China), CCK-8 cell viability assay kit (#PF00004, Proteintech, Wuhan, China), rabbit cleaved caspase-3 antibody (#9664, Cell Signaling Technology, Massachusetts, USA), rabbit anti-caspase-3 antibody (#66470-2-Ig, Proteintech, Wuhan, China), rabbit anti-Bax antibody (#WL01637, Wanle, Shenyang, China), rabbit anti-B-cell lymphoma-2 (Bcl-2) antibody (#WL01556, Wanlei, Shenyang, China), rabbit anti-JAK2 antibody (#3230S, Cell Signaling Technology, Massachusetts, USA), rabbit anti-p-JAK2 antibody (#ab32101, Abcam, Cambridge, UK), rabbit anti-STAT3 antibody (#12640, Cell Signaling Technology, Massachusetts, USA), Rabbit p-STAT3 antibody (#9145S, Cell Signaling Technology, Massachusetts, USA), murine anti-β-actin antibody (#66009-1-Ig, Proteintech, Wuhan, China), murine anti-NeuN antibody (#66,836–1-Ig, Proteintech, Wuhan, China), horseradish peroxidase (HPR)-labelled anti-rabbit IgG antibody (#SA00001-2, Proteintech, Wuhan, China), HPR-labelled anti-mouse IgG antibody (#SA00001-1, Proteintech, Wuhan, China), DAPI-containing fluorescent coated tablets (#S2110, Solarbio, Beijing, China), Cy3-labelled goat anti-rabbit IgG antibody (#SA00009-2, Proteintech, Wuhan, China), AF488-labelled goat anti-mouse IgG antibody (#SA00013-1, Proteintech, Wuhan, China). .. A cell incubator (#51033564, Thermo Fisher Scientific, Waltham, USA), hypoxia chamber (#27310, STEMCELL, Columbia, USA), vertical electrophoresis apparatus (#1658033, Bio-Rad, California, USA), semi-dry rotary membrane apparatus (#1703940, Bio-Rad, California, USA), imaging apparatus (#1708280, Bio-Rad, California, USA), and an inverted fluorescence microscope (#LSM 880, Zeiss, Jena, Germany).

    Article Title: BAG3 promotes proliferation and migration of arterial smooth muscle cells by regulating STAT3 phosphorylation in diabetic vascular remodeling.
    Article Snippet: .. Rabbit-anti-BAG3 antibody (10599-1-AP, Proteintech, WB: 1:2000; IP: 1:1000; IF: 1:100), rabbit-anti-MMP2 antibody (10373-2-AP, Proteintech, WB: 1:1000), rabbit-anti-MMP9 antibody (10375-2-AP, Proteintech, WB: 1:1000), rabbit-anti-PCNA antibody (10205-2-AP, Proteintech, WB: 1:1000), mouse-anti-Tubulin antibody (66031-1-Ig, Proteintech, WB: 1:1000), mouse-antiGAPDH antibody (60004-1-Ig, Proteintech, WB: 1:1000), mouse-anti-Flag antibody (66008-4-Ig, Proteintech, WB: 1:1000; IP: 1:1000), rabbit-anti-JAK2 antibody (3230 S, CST, WB: 1:1000), rabbit-anti-p-JAK2 antibody (3776 S, CST, WB: 1:1000), mouse-anti-STAT3 antibody (9139 S, CST, WB: 1:1000; IP: 1:1000), rabbit-anti-p-STAT3(705) antibody (9145 S, CST, WB: 1:1000; IF: 1:100), rabbit-anti-p-STAT3(727) antibody (94,994 S, CST, WB: 1:1000; IF: 1:100), mouse-anti-ERK1/2 antibody (sc514,302, Santa, WB: 1:1000), mouse-anti-p-ERK1/2 antibody (sc-81,492, Santa, WB: 1:1000), mouse-anti-GATA3 antibody (66400-1-Ig, Proteintech, WB: 1:1000), HRP goat-anti-rabbit IgG antibody (A21020, Abbkine, WB: 1:10000), HRP goat-anti-mouse IgG antibody (A21010, Abbkine, WB: 1:10000), Rhodamine(TRITIC) conjugated goat-anti-rabbit IgG(H + L) (SA00007-2, Proteintech, IF: 1:200), Protein A/G magnetic Beads (Cat#B23202, Biotool), AntiFade Mounting Medium (Beyotime), CCK-8 cell viability kit (B34304, Bimake), Higene transfection reagent (C1506, APPLYGEN), Jet kit (101,000,046, Polyplus), protease inhibitor (B14001, Bimake), phosphatase inhibitors (B15001, Bimake), SH-4-54 (S7337, Selleck), U0126-EtOH (S1102, Selleck). ..

    Transfection:

    Article Title: BAG3 promotes proliferation and migration of arterial smooth muscle cells by regulating STAT3 phosphorylation in diabetic vascular remodeling
    Article Snippet: .. Rabbit-anti-BAG3 antibody (10599-1-AP, Proteintech, WB: 1:2000; IP: 1:1000; IF: 1:100), rabbit-anti-MMP2 antibody (10373-2-AP, Proteintech, WB: 1:1000), rabbit-anti-MMP9 antibody (10375-2-AP, Proteintech, WB: 1:1000), rabbit-anti-PCNA antibody (10205-2-AP, Proteintech, WB: 1:1000), mouse-anti-Tubulin antibody (66031-1-Ig, Proteintech, WB: 1:1000), mouse-anti-GAPDH antibody (60004-1-Ig, Proteintech, WB: 1:1000), mouse-anti-Flag antibody (66008-4-Ig, Proteintech, WB: 1:1000; IP: 1:1000), rabbit-anti-JAK2 antibody (3230 S, CST, WB: 1:1000), rabbit-anti-p-JAK2 antibody (3776 S, CST, WB: 1:1000), mouse-anti-STAT3 antibody (9139 S, CST, WB: 1:1000; IP: 1:1000), rabbit-anti-p-STAT3(705) antibody (9145 S, CST, WB: 1:1000; IF: 1:100), rabbit-anti-p-STAT3(727) antibody (94,994 S, CST, WB: 1:1000; IF: 1:100), mouse-anti-ERK1/2 antibody (sc-514,302, Santa, WB: 1:1000), mouse-anti-p-ERK1/2 antibody (sc-81,492, Santa, WB: 1:1000), mouse-anti-GATA3 antibody (66400-1-Ig, Proteintech, WB: 1:1000), HRP goat-anti-rabbit IgG antibody (A21020, Abbkine, WB: 1:10000), HRP goat-anti-mouse IgG antibody (A21010, Abbkine, WB: 1:10000), Rhodamine(TRITIC) conjugated goat-anti-rabbit IgG(H + L) (SA00007-2, Proteintech, IF: 1:200), Protein A/G magnetic Beads (Cat#B23202, Biotool), AntiFade Mounting Medium (Beyotime), CCK-8 cell viability kit (B34304, Bimake), Higene transfection reagent (C1506, APPLYGEN), Jet kit (101,000,046, Polyplus), protease inhibitor (B14001, Bimake), phosphatase inhibitors (B15001, Bimake), SH-4-54 (S7337, Selleck), U0126-EtOH (S1102, Selleck). ..

    Article Title: BAG3 promotes proliferation and migration of arterial smooth muscle cells by regulating STAT3 phosphorylation in diabetic vascular remodeling
    Article Snippet: .. 2.17 Antibodies and reagents Page 7/24 Rabbit-anti-BAG3 antibody (10599-1-AP, Proteintech, WB: 1:2000; IP: 1:1000; IF: 1:100), rabbit-anti-MMP2 antibody (10373-2-AP, Proteintech, WB: 1:1000), rabbit-anti-MMP9 antibody (10375-2-AP, Proteintech, WB: 1:1000), rabbit-anti-PCNA antibody (10205-2-AP, Proteintech, WB: 1:1000), mouse-anti-Tubulin antibody (66031-1-Ig, Proteintech, WB: 1:1000), mouse-anti-GAPDH antibody (60004-1-Ig, Proteintech, WB: 1:1000), mouse-anti-Flag antibody (66008-4-Ig, Proteintech, WB: 1:1000; IP: 1:1000), rabbit-anti-JAK2 antibody (3230S, CST, WB: 1:1000), rabbit-anti-p-JAK2 antibody (3776S, CST, WB: 1:1000), mouse-anti-STAT3 antibody (9139S, CST, WB: 1:1000; IP: 1:1000), rabbit-anti-p-STAT3(705) antibody (9145S, CST, WB: 1:1000), rabbit-anti-p-STAT3(727) antibody (94994S, CST, WB: 1:1000), mouse-anti-ERK1/2 antibody (sc514302, Santa, WB: 1:1000), mouse-anti-p-ERK1/2 antibody (sc-81492, Santa, WB: 1:1000), mouse-antiGATA3 antibody (66400-1-Ig, Proteintech, WB: 1:1000), HRP goat-anti-rabbit IgG antibody (A21020, Abbkine, WB: 1:10000), HRP goat-anti-mouse IgG antibody (A21010, Abbkine, WB: 1:10000), Rhodamine(TRITIC) conjugated goat-anti-rabbit IgG(H + L) (SA00007-2, Proteintech, IF: 1:200), Protein A/G magnetic Beads (Cat#B23202, Biotool), AntiFade Mounting Medium (Beyotime), CCK-8 cell viability kit (B34304, Bimake), Higene transfection reagent (C1506, APPLYGEN), Jet kit (101000046, Polyplus), protease inhibitor (B14001, Bimake), phosphatase inhibitors (B15001, Bimake), SH-4-54 (S7337, Selleck), U0126-EtOH (S1102, Selleck). ..

    Article Title: BAG3 promotes proliferation and migration of arterial smooth muscle cells by regulating STAT3 phosphorylation in diabetic vascular remodeling.
    Article Snippet: .. Rabbit-anti-BAG3 antibody (10599-1-AP, Proteintech, WB: 1:2000; IP: 1:1000; IF: 1:100), rabbit-anti-MMP2 antibody (10373-2-AP, Proteintech, WB: 1:1000), rabbit-anti-MMP9 antibody (10375-2-AP, Proteintech, WB: 1:1000), rabbit-anti-PCNA antibody (10205-2-AP, Proteintech, WB: 1:1000), mouse-anti-Tubulin antibody (66031-1-Ig, Proteintech, WB: 1:1000), mouse-antiGAPDH antibody (60004-1-Ig, Proteintech, WB: 1:1000), mouse-anti-Flag antibody (66008-4-Ig, Proteintech, WB: 1:1000; IP: 1:1000), rabbit-anti-JAK2 antibody (3230 S, CST, WB: 1:1000), rabbit-anti-p-JAK2 antibody (3776 S, CST, WB: 1:1000), mouse-anti-STAT3 antibody (9139 S, CST, WB: 1:1000; IP: 1:1000), rabbit-anti-p-STAT3(705) antibody (9145 S, CST, WB: 1:1000; IF: 1:100), rabbit-anti-p-STAT3(727) antibody (94,994 S, CST, WB: 1:1000; IF: 1:100), mouse-anti-ERK1/2 antibody (sc514,302, Santa, WB: 1:1000), mouse-anti-p-ERK1/2 antibody (sc-81,492, Santa, WB: 1:1000), mouse-anti-GATA3 antibody (66400-1-Ig, Proteintech, WB: 1:1000), HRP goat-anti-rabbit IgG antibody (A21020, Abbkine, WB: 1:10000), HRP goat-anti-mouse IgG antibody (A21010, Abbkine, WB: 1:10000), Rhodamine(TRITIC) conjugated goat-anti-rabbit IgG(H + L) (SA00007-2, Proteintech, IF: 1:200), Protein A/G magnetic Beads (Cat#B23202, Biotool), AntiFade Mounting Medium (Beyotime), CCK-8 cell viability kit (B34304, Bimake), Higene transfection reagent (C1506, APPLYGEN), Jet kit (101,000,046, Polyplus), protease inhibitor (B14001, Bimake), phosphatase inhibitors (B15001, Bimake), SH-4-54 (S7337, Selleck), U0126-EtOH (S1102, Selleck). ..

    Protease Inhibitor:

    Article Title: BAG3 promotes proliferation and migration of arterial smooth muscle cells by regulating STAT3 phosphorylation in diabetic vascular remodeling
    Article Snippet: .. Rabbit-anti-BAG3 antibody (10599-1-AP, Proteintech, WB: 1:2000; IP: 1:1000; IF: 1:100), rabbit-anti-MMP2 antibody (10373-2-AP, Proteintech, WB: 1:1000), rabbit-anti-MMP9 antibody (10375-2-AP, Proteintech, WB: 1:1000), rabbit-anti-PCNA antibody (10205-2-AP, Proteintech, WB: 1:1000), mouse-anti-Tubulin antibody (66031-1-Ig, Proteintech, WB: 1:1000), mouse-anti-GAPDH antibody (60004-1-Ig, Proteintech, WB: 1:1000), mouse-anti-Flag antibody (66008-4-Ig, Proteintech, WB: 1:1000; IP: 1:1000), rabbit-anti-JAK2 antibody (3230 S, CST, WB: 1:1000), rabbit-anti-p-JAK2 antibody (3776 S, CST, WB: 1:1000), mouse-anti-STAT3 antibody (9139 S, CST, WB: 1:1000; IP: 1:1000), rabbit-anti-p-STAT3(705) antibody (9145 S, CST, WB: 1:1000; IF: 1:100), rabbit-anti-p-STAT3(727) antibody (94,994 S, CST, WB: 1:1000; IF: 1:100), mouse-anti-ERK1/2 antibody (sc-514,302, Santa, WB: 1:1000), mouse-anti-p-ERK1/2 antibody (sc-81,492, Santa, WB: 1:1000), mouse-anti-GATA3 antibody (66400-1-Ig, Proteintech, WB: 1:1000), HRP goat-anti-rabbit IgG antibody (A21020, Abbkine, WB: 1:10000), HRP goat-anti-mouse IgG antibody (A21010, Abbkine, WB: 1:10000), Rhodamine(TRITIC) conjugated goat-anti-rabbit IgG(H + L) (SA00007-2, Proteintech, IF: 1:200), Protein A/G magnetic Beads (Cat#B23202, Biotool), AntiFade Mounting Medium (Beyotime), CCK-8 cell viability kit (B34304, Bimake), Higene transfection reagent (C1506, APPLYGEN), Jet kit (101,000,046, Polyplus), protease inhibitor (B14001, Bimake), phosphatase inhibitors (B15001, Bimake), SH-4-54 (S7337, Selleck), U0126-EtOH (S1102, Selleck). ..

    Article Title: BAG3 promotes proliferation and migration of arterial smooth muscle cells by regulating STAT3 phosphorylation in diabetic vascular remodeling
    Article Snippet: .. 2.17 Antibodies and reagents Page 7/24 Rabbit-anti-BAG3 antibody (10599-1-AP, Proteintech, WB: 1:2000; IP: 1:1000; IF: 1:100), rabbit-anti-MMP2 antibody (10373-2-AP, Proteintech, WB: 1:1000), rabbit-anti-MMP9 antibody (10375-2-AP, Proteintech, WB: 1:1000), rabbit-anti-PCNA antibody (10205-2-AP, Proteintech, WB: 1:1000), mouse-anti-Tubulin antibody (66031-1-Ig, Proteintech, WB: 1:1000), mouse-anti-GAPDH antibody (60004-1-Ig, Proteintech, WB: 1:1000), mouse-anti-Flag antibody (66008-4-Ig, Proteintech, WB: 1:1000; IP: 1:1000), rabbit-anti-JAK2 antibody (3230S, CST, WB: 1:1000), rabbit-anti-p-JAK2 antibody (3776S, CST, WB: 1:1000), mouse-anti-STAT3 antibody (9139S, CST, WB: 1:1000; IP: 1:1000), rabbit-anti-p-STAT3(705) antibody (9145S, CST, WB: 1:1000), rabbit-anti-p-STAT3(727) antibody (94994S, CST, WB: 1:1000), mouse-anti-ERK1/2 antibody (sc514302, Santa, WB: 1:1000), mouse-anti-p-ERK1/2 antibody (sc-81492, Santa, WB: 1:1000), mouse-antiGATA3 antibody (66400-1-Ig, Proteintech, WB: 1:1000), HRP goat-anti-rabbit IgG antibody (A21020, Abbkine, WB: 1:10000), HRP goat-anti-mouse IgG antibody (A21010, Abbkine, WB: 1:10000), Rhodamine(TRITIC) conjugated goat-anti-rabbit IgG(H + L) (SA00007-2, Proteintech, IF: 1:200), Protein A/G magnetic Beads (Cat#B23202, Biotool), AntiFade Mounting Medium (Beyotime), CCK-8 cell viability kit (B34304, Bimake), Higene transfection reagent (C1506, APPLYGEN), Jet kit (101000046, Polyplus), protease inhibitor (B14001, Bimake), phosphatase inhibitors (B15001, Bimake), SH-4-54 (S7337, Selleck), U0126-EtOH (S1102, Selleck). ..

    Article Title: BAG3 promotes proliferation and migration of arterial smooth muscle cells by regulating STAT3 phosphorylation in diabetic vascular remodeling.
    Article Snippet: .. Rabbit-anti-BAG3 antibody (10599-1-AP, Proteintech, WB: 1:2000; IP: 1:1000; IF: 1:100), rabbit-anti-MMP2 antibody (10373-2-AP, Proteintech, WB: 1:1000), rabbit-anti-MMP9 antibody (10375-2-AP, Proteintech, WB: 1:1000), rabbit-anti-PCNA antibody (10205-2-AP, Proteintech, WB: 1:1000), mouse-anti-Tubulin antibody (66031-1-Ig, Proteintech, WB: 1:1000), mouse-antiGAPDH antibody (60004-1-Ig, Proteintech, WB: 1:1000), mouse-anti-Flag antibody (66008-4-Ig, Proteintech, WB: 1:1000; IP: 1:1000), rabbit-anti-JAK2 antibody (3230 S, CST, WB: 1:1000), rabbit-anti-p-JAK2 antibody (3776 S, CST, WB: 1:1000), mouse-anti-STAT3 antibody (9139 S, CST, WB: 1:1000; IP: 1:1000), rabbit-anti-p-STAT3(705) antibody (9145 S, CST, WB: 1:1000; IF: 1:100), rabbit-anti-p-STAT3(727) antibody (94,994 S, CST, WB: 1:1000; IF: 1:100), mouse-anti-ERK1/2 antibody (sc514,302, Santa, WB: 1:1000), mouse-anti-p-ERK1/2 antibody (sc-81,492, Santa, WB: 1:1000), mouse-anti-GATA3 antibody (66400-1-Ig, Proteintech, WB: 1:1000), HRP goat-anti-rabbit IgG antibody (A21020, Abbkine, WB: 1:10000), HRP goat-anti-mouse IgG antibody (A21010, Abbkine, WB: 1:10000), Rhodamine(TRITIC) conjugated goat-anti-rabbit IgG(H + L) (SA00007-2, Proteintech, IF: 1:200), Protein A/G magnetic Beads (Cat#B23202, Biotool), AntiFade Mounting Medium (Beyotime), CCK-8 cell viability kit (B34304, Bimake), Higene transfection reagent (C1506, APPLYGEN), Jet kit (101,000,046, Polyplus), protease inhibitor (B14001, Bimake), phosphatase inhibitors (B15001, Bimake), SH-4-54 (S7337, Selleck), U0126-EtOH (S1102, Selleck). ..

    Lysis:

    Article Title: Effect of spirocyclopiperazinium salt compound LXM-15 on spinal nerve injury in rats.
    Article Snippet: Eur J Pain.. 2024;28:297–309. wileyonlinelibrary.com/journal/ejp | 297© 2023 European Pain Federation - EFIC ®.. Tian Yu Wang and Ying Ying Liang contributed equally to this work.

    Bicinchoninic Acid Protein Assay:

    Article Title: Effect of spirocyclopiperazinium salt compound LXM-15 on spinal nerve injury in rats.
    Article Snippet: Eur J Pain.. 2024;28:297–309. wileyonlinelibrary.com/journal/ejp | 297© 2023 European Pain Federation - EFIC ®.. Tian Yu Wang and Ying Ying Liang contributed equally to this work.

    Binding Assay:

    Article Title: Effect of spirocyclopiperazinium salt compound LXM-15 on spinal nerve injury in rats.
    Article Snippet: Eur J Pain.. 2024;28:297–309. wileyonlinelibrary.com/journal/ejp | 297© 2023 European Pain Federation - EFIC ®.. Tian Yu Wang and Ying Ying Liang contributed equally to this work.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Effect of spirocyclopiperazinium salt compound LXM-15 on spinal nerve injury in rats.
    Article Snippet: Eur J Pain.. 2024;28:297–309. wileyonlinelibrary.com/journal/ejp | 297© 2023 European Pain Federation - EFIC ®.. Tian Yu Wang and Ying Ying Liang contributed equally to this work.

    Incubation:

    Article Title: CCDC80 Protects against Aortic Dissection and Rupture by Maintaining the Contractile Smooth Muscle Cell Phenotype.
    Article Snippet: Approximately 300 μg of the lysates were incubated with 15 μL of Protein A/G magnetic beads (88802; Thermo Fisher Scientific) at 4 °C overnight. .. Next, protein-magnetic beads were incubated with rabbit anti-JAK2 antibody, rabbit anti-CCDC80 antibody, or anti-IgG (2729; Cell Signaling Technology) at 4 °C overnight. ..

    Article Title: Allicin Ameliorated High-glucose Peritoneal Dialysis Solution-induced Peritoneal Fibrosis in Rats via the JAK2/STAT3 Signaling Pathway.
    Article Snippet: Peritoneal fibrosis (PF) is one of the most serious complications of peritoneal dialysis (PD) and is the greatest obstacle to the clinical application of PD.. Chinese herbal monomers have been effective in the prevention and treatment of PF.. The aim of this study was to observe the effect of allicin on PF in rats induced by high glucose and to investigate its molecular mechanism of action.

    Cell Culture:

    Article Title: Effect of scutellarin on BV-2 microglial-mediated apoptosis in PC12 cells via JAK2/STAT3 signalling pathway.
    Article Snippet: .. Scutellarin (#THT033, Ronghe Medical Technology, Shanghai, China), F12/DMEM 1:1 high sugar medium (#C3130-0500, BI, Shanghai, China ), sugar-free culture medium (#C3122-500, BI, Shanghai, China), cell culture level anhydrous glucose (#G8150, Solarbio, Beijing, China), TUNEL kit (#PF00006, Proteintech, Wuhan, China), CCK-8 cell viability assay kit (#PF00004, Proteintech, Wuhan, China), rabbit cleaved caspase-3 antibody (#9664, Cell Signaling Technology, Massachusetts, USA), rabbit anti-caspase-3 antibody (#66470-2-Ig, Proteintech, Wuhan, China), rabbit anti-Bax antibody (#WL01637, Wanle, Shenyang, China), rabbit anti-B-cell lymphoma-2 (Bcl-2) antibody (#WL01556, Wanlei, Shenyang, China), rabbit anti-JAK2 antibody (#3230S, Cell Signaling Technology, Massachusetts, USA), rabbit anti-p-JAK2 antibody (#ab32101, Abcam, Cambridge, UK), rabbit anti-STAT3 antibody (#12640, Cell Signaling Technology, Massachusetts, USA), Rabbit p-STAT3 antibody (#9145S, Cell Signaling Technology, Massachusetts, USA), murine anti-β-actin antibody (#66009-1-Ig, Proteintech, Wuhan, China), murine anti-NeuN antibody (#66,836–1-Ig, Proteintech, Wuhan, China), horseradish peroxidase (HPR)-labelled anti-rabbit IgG antibody (#SA00001-2, Proteintech, Wuhan, China), HPR-labelled anti-mouse IgG antibody (#SA00001-1, Proteintech, Wuhan, China), DAPI-containing fluorescent coated tablets (#S2110, Solarbio, Beijing, China), Cy3-labelled goat anti-rabbit IgG antibody (#SA00009-2, Proteintech, Wuhan, China), AF488-labelled goat anti-mouse IgG antibody (#SA00013-1, Proteintech, Wuhan, China). .. A cell incubator (#51033564, Thermo Fisher Scientific, Waltham, USA), hypoxia chamber (#27310, STEMCELL, Columbia, USA), vertical electrophoresis apparatus (#1658033, Bio-Rad, California, USA), semi-dry rotary membrane apparatus (#1703940, Bio-Rad, California, USA), imaging apparatus (#1708280, Bio-Rad, California, USA), and an inverted fluorescence microscope (#LSM 880, Zeiss, Jena, Germany).

    TUNEL Assay:

    Article Title: Effect of scutellarin on BV-2 microglial-mediated apoptosis in PC12 cells via JAK2/STAT3 signalling pathway.
    Article Snippet: .. Scutellarin (#THT033, Ronghe Medical Technology, Shanghai, China), F12/DMEM 1:1 high sugar medium (#C3130-0500, BI, Shanghai, China ), sugar-free culture medium (#C3122-500, BI, Shanghai, China), cell culture level anhydrous glucose (#G8150, Solarbio, Beijing, China), TUNEL kit (#PF00006, Proteintech, Wuhan, China), CCK-8 cell viability assay kit (#PF00004, Proteintech, Wuhan, China), rabbit cleaved caspase-3 antibody (#9664, Cell Signaling Technology, Massachusetts, USA), rabbit anti-caspase-3 antibody (#66470-2-Ig, Proteintech, Wuhan, China), rabbit anti-Bax antibody (#WL01637, Wanle, Shenyang, China), rabbit anti-B-cell lymphoma-2 (Bcl-2) antibody (#WL01556, Wanlei, Shenyang, China), rabbit anti-JAK2 antibody (#3230S, Cell Signaling Technology, Massachusetts, USA), rabbit anti-p-JAK2 antibody (#ab32101, Abcam, Cambridge, UK), rabbit anti-STAT3 antibody (#12640, Cell Signaling Technology, Massachusetts, USA), Rabbit p-STAT3 antibody (#9145S, Cell Signaling Technology, Massachusetts, USA), murine anti-β-actin antibody (#66009-1-Ig, Proteintech, Wuhan, China), murine anti-NeuN antibody (#66,836–1-Ig, Proteintech, Wuhan, China), horseradish peroxidase (HPR)-labelled anti-rabbit IgG antibody (#SA00001-2, Proteintech, Wuhan, China), HPR-labelled anti-mouse IgG antibody (#SA00001-1, Proteintech, Wuhan, China), DAPI-containing fluorescent coated tablets (#S2110, Solarbio, Beijing, China), Cy3-labelled goat anti-rabbit IgG antibody (#SA00009-2, Proteintech, Wuhan, China), AF488-labelled goat anti-mouse IgG antibody (#SA00013-1, Proteintech, Wuhan, China). .. A cell incubator (#51033564, Thermo Fisher Scientific, Waltham, USA), hypoxia chamber (#27310, STEMCELL, Columbia, USA), vertical electrophoresis apparatus (#1658033, Bio-Rad, California, USA), semi-dry rotary membrane apparatus (#1703940, Bio-Rad, California, USA), imaging apparatus (#1708280, Bio-Rad, California, USA), and an inverted fluorescence microscope (#LSM 880, Zeiss, Jena, Germany).

    Viability Assay:

    Article Title: Effect of scutellarin on BV-2 microglial-mediated apoptosis in PC12 cells via JAK2/STAT3 signalling pathway.
    Article Snippet: .. Scutellarin (#THT033, Ronghe Medical Technology, Shanghai, China), F12/DMEM 1:1 high sugar medium (#C3130-0500, BI, Shanghai, China ), sugar-free culture medium (#C3122-500, BI, Shanghai, China), cell culture level anhydrous glucose (#G8150, Solarbio, Beijing, China), TUNEL kit (#PF00006, Proteintech, Wuhan, China), CCK-8 cell viability assay kit (#PF00004, Proteintech, Wuhan, China), rabbit cleaved caspase-3 antibody (#9664, Cell Signaling Technology, Massachusetts, USA), rabbit anti-caspase-3 antibody (#66470-2-Ig, Proteintech, Wuhan, China), rabbit anti-Bax antibody (#WL01637, Wanle, Shenyang, China), rabbit anti-B-cell lymphoma-2 (Bcl-2) antibody (#WL01556, Wanlei, Shenyang, China), rabbit anti-JAK2 antibody (#3230S, Cell Signaling Technology, Massachusetts, USA), rabbit anti-p-JAK2 antibody (#ab32101, Abcam, Cambridge, UK), rabbit anti-STAT3 antibody (#12640, Cell Signaling Technology, Massachusetts, USA), Rabbit p-STAT3 antibody (#9145S, Cell Signaling Technology, Massachusetts, USA), murine anti-β-actin antibody (#66009-1-Ig, Proteintech, Wuhan, China), murine anti-NeuN antibody (#66,836–1-Ig, Proteintech, Wuhan, China), horseradish peroxidase (HPR)-labelled anti-rabbit IgG antibody (#SA00001-2, Proteintech, Wuhan, China), HPR-labelled anti-mouse IgG antibody (#SA00001-1, Proteintech, Wuhan, China), DAPI-containing fluorescent coated tablets (#S2110, Solarbio, Beijing, China), Cy3-labelled goat anti-rabbit IgG antibody (#SA00009-2, Proteintech, Wuhan, China), AF488-labelled goat anti-mouse IgG antibody (#SA00013-1, Proteintech, Wuhan, China). .. A cell incubator (#51033564, Thermo Fisher Scientific, Waltham, USA), hypoxia chamber (#27310, STEMCELL, Columbia, USA), vertical electrophoresis apparatus (#1658033, Bio-Rad, California, USA), semi-dry rotary membrane apparatus (#1703940, Bio-Rad, California, USA), imaging apparatus (#1708280, Bio-Rad, California, USA), and an inverted fluorescence microscope (#LSM 880, Zeiss, Jena, Germany).

    Membrane:

    Article Title: Allicin Ameliorated High-glucose Peritoneal Dialysis Solution-induced Peritoneal Fibrosis in Rats via the JAK2/STAT3 Signaling Pathway.
    Article Snippet: Peritoneal fibrosis (PF) is one of the most serious complications of peritoneal dialysis (PD) and is the greatest obstacle to the clinical application of PD.. Chinese herbal monomers have been effective in the prevention and treatment of PF.. The aim of this study was to observe the effect of allicin on PF in rats induced by high glucose and to investigate its molecular mechanism of action.



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    Effects of STG on the JAK2/STAT3 signaling pathway. ( A ) Molecular docking results of JAK2 and STAT3 with STG. ( B ) Expression of JAK2/STAT3 signaling pathway proteins. ( C ) Statistical analysis of JAK2/STAT3 signaling pathway protein expression. Data are shown as the mean ± SD of three independent experiments. Compared with the STG (−) AG490 (−) group, * p < 0.05, *** p < 0.001; compared with the STG (+) AG490 (−) group, ## p < 0.01, ### p < 0.001.

    Journal: Current Issues in Molecular Biology

    Article Title: The Mechanism of Action of Stigmasterol in Bone Formation in Osteoporosis

    doi: 10.3390/cimb48030337

    Figure Lengend Snippet: Effects of STG on the JAK2/STAT3 signaling pathway. ( A ) Molecular docking results of JAK2 and STAT3 with STG. ( B ) Expression of JAK2/STAT3 signaling pathway proteins. ( C ) Statistical analysis of JAK2/STAT3 signaling pathway protein expression. Data are shown as the mean ± SD of three independent experiments. Compared with the STG (−) AG490 (−) group, * p < 0.05, *** p < 0.001; compared with the STG (+) AG490 (−) group, ## p < 0.01, ### p < 0.001.

    Article Snippet: The primary antibodies utilized in this study included β-actin (ZSGB-BIO, #TA-09, Beijing, China), Osteocalcin (ABclonal, #A20800, Wuhan, China), RUNX2 (ABclonal, #A11753), STAT3 (ABclonal, #A1192), Phospho-STAT3 (Tyr705) (ABclonal, #AP0705), JAK2 (Cell Signaling Technology, #3230), and Phospho-JAK2 (Tyr1007/1008) (Cell Signaling Technology, #3776, Danvers, MA, USA).

    Techniques: Expressing

    The effect of STG on osteogenic differentiation via the JAK2/STAT3 signaling pathway. ( A ) Expression of osteogenic marker proteins. ( B ) Statistical analysis of osteogenic marker protein expression. ( C ) ALP and ARS staining. Data are shown as the mean ± SD of three independent experiments. Compared with the STG (−) AG490 (−) group, * p < 0.05, ** p < 0.01; compared with the STG (+) AG490 (−) group, ### p < 0.001.

    Journal: Current Issues in Molecular Biology

    Article Title: The Mechanism of Action of Stigmasterol in Bone Formation in Osteoporosis

    doi: 10.3390/cimb48030337

    Figure Lengend Snippet: The effect of STG on osteogenic differentiation via the JAK2/STAT3 signaling pathway. ( A ) Expression of osteogenic marker proteins. ( B ) Statistical analysis of osteogenic marker protein expression. ( C ) ALP and ARS staining. Data are shown as the mean ± SD of three independent experiments. Compared with the STG (−) AG490 (−) group, * p < 0.05, ** p < 0.01; compared with the STG (+) AG490 (−) group, ### p < 0.001.

    Article Snippet: The primary antibodies utilized in this study included β-actin (ZSGB-BIO, #TA-09, Beijing, China), Osteocalcin (ABclonal, #A20800, Wuhan, China), RUNX2 (ABclonal, #A11753), STAT3 (ABclonal, #A1192), Phospho-STAT3 (Tyr705) (ABclonal, #AP0705), JAK2 (Cell Signaling Technology, #3230), and Phospho-JAK2 (Tyr1007/1008) (Cell Signaling Technology, #3776, Danvers, MA, USA).

    Techniques: Expressing, Marker, Staining

    Schematic diagram of the role of STG in the JAK2/STAT3 pathway.

    Journal: Current Issues in Molecular Biology

    Article Title: The Mechanism of Action of Stigmasterol in Bone Formation in Osteoporosis

    doi: 10.3390/cimb48030337

    Figure Lengend Snippet: Schematic diagram of the role of STG in the JAK2/STAT3 pathway.

    Article Snippet: The primary antibodies utilized in this study included β-actin (ZSGB-BIO, #TA-09, Beijing, China), Osteocalcin (ABclonal, #A20800, Wuhan, China), RUNX2 (ABclonal, #A11753), STAT3 (ABclonal, #A1192), Phospho-STAT3 (Tyr705) (ABclonal, #AP0705), JAK2 (Cell Signaling Technology, #3230), and Phospho-JAK2 (Tyr1007/1008) (Cell Signaling Technology, #3776, Danvers, MA, USA).

    Techniques:

    Effects of STG on the JAK2/STAT3 signaling pathway. ( A ) Molecular docking results of JAK2 and STAT3 with STG. ( B ) Expression of JAK2/STAT3 signaling pathway proteins. ( C ) Statistical analysis of JAK2/STAT3 signaling pathway protein expression. Data are shown as the mean ± SD of three independent experiments. Compared with the STG (−) AG490 (−) group, * p < 0.05, *** p < 0.001; compared with the STG (+) AG490 (−) group, ## p < 0.01, ### p < 0.001.

    Journal: Current Issues in Molecular Biology

    Article Title: The Mechanism of Action of Stigmasterol in Bone Formation in Osteoporosis

    doi: 10.3390/cimb48030337

    Figure Lengend Snippet: Effects of STG on the JAK2/STAT3 signaling pathway. ( A ) Molecular docking results of JAK2 and STAT3 with STG. ( B ) Expression of JAK2/STAT3 signaling pathway proteins. ( C ) Statistical analysis of JAK2/STAT3 signaling pathway protein expression. Data are shown as the mean ± SD of three independent experiments. Compared with the STG (−) AG490 (−) group, * p < 0.05, *** p < 0.001; compared with the STG (+) AG490 (−) group, ## p < 0.01, ### p < 0.001.

    Article Snippet: The primary antibodies utilized in this study included β-actin (ZSGB-BIO, #TA-09, Beijing, China), Osteocalcin (ABclonal, #A20800, Wuhan, China), RUNX2 (ABclonal, #A11753), STAT3 (ABclonal, #A1192), Phospho-STAT3 (Tyr705) (ABclonal, #AP0705), JAK2 (Cell Signaling Technology, #3230), and Phospho-JAK2 (Tyr1007/1008) (Cell Signaling Technology, #3776, Danvers, MA, USA).

    Techniques: Expressing

    The effect of STG on osteogenic differentiation via the JAK2/STAT3 signaling pathway. ( A ) Expression of osteogenic marker proteins. ( B ) Statistical analysis of osteogenic marker protein expression. ( C ) ALP and ARS staining. Data are shown as the mean ± SD of three independent experiments. Compared with the STG (−) AG490 (−) group, * p < 0.05, ** p < 0.01; compared with the STG (+) AG490 (−) group, ### p < 0.001.

    Journal: Current Issues in Molecular Biology

    Article Title: The Mechanism of Action of Stigmasterol in Bone Formation in Osteoporosis

    doi: 10.3390/cimb48030337

    Figure Lengend Snippet: The effect of STG on osteogenic differentiation via the JAK2/STAT3 signaling pathway. ( A ) Expression of osteogenic marker proteins. ( B ) Statistical analysis of osteogenic marker protein expression. ( C ) ALP and ARS staining. Data are shown as the mean ± SD of three independent experiments. Compared with the STG (−) AG490 (−) group, * p < 0.05, ** p < 0.01; compared with the STG (+) AG490 (−) group, ### p < 0.001.

    Article Snippet: The primary antibodies utilized in this study included β-actin (ZSGB-BIO, #TA-09, Beijing, China), Osteocalcin (ABclonal, #A20800, Wuhan, China), RUNX2 (ABclonal, #A11753), STAT3 (ABclonal, #A1192), Phospho-STAT3 (Tyr705) (ABclonal, #AP0705), JAK2 (Cell Signaling Technology, #3230), and Phospho-JAK2 (Tyr1007/1008) (Cell Signaling Technology, #3776, Danvers, MA, USA).

    Techniques: Expressing, Marker, Staining

    Schematic diagram of the role of STG in the JAK2/STAT3 pathway.

    Journal: Current Issues in Molecular Biology

    Article Title: The Mechanism of Action of Stigmasterol in Bone Formation in Osteoporosis

    doi: 10.3390/cimb48030337

    Figure Lengend Snippet: Schematic diagram of the role of STG in the JAK2/STAT3 pathway.

    Article Snippet: The primary antibodies utilized in this study included β-actin (ZSGB-BIO, #TA-09, Beijing, China), Osteocalcin (ABclonal, #A20800, Wuhan, China), RUNX2 (ABclonal, #A11753), STAT3 (ABclonal, #A1192), Phospho-STAT3 (Tyr705) (ABclonal, #AP0705), JAK2 (Cell Signaling Technology, #3230), and Phospho-JAK2 (Tyr1007/1008) (Cell Signaling Technology, #3776, Danvers, MA, USA).

    Techniques: